Histone H2AX Antibody (CL5157) - BSA Free

Staining of MCF7 cells using the Anti-H2AFX monoclonal antibody, showing specific staining in nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.

Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.

Staining of human kidney shows strong nuclear positivity in renal tubules cells.

Staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL5157
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2AX Antibody (CL5157) - BSA Free Summary

• Description: Novus Biologicals Mouse Histone H2AX Antibody (CL5157) - BSA Free (NBP3-21157) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P16104, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2AX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:20000 - 1:50000
  • Immunohistochemistry-Paraffin 1:20000 - 1:50000
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Histone H2AX Antibody (CL5157) - BSA Free

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AX