Histone Deacetylase 2/HDAC2 [p Ser394] Antibody - Azide Free

Western Blot: HDAC2 [p Ser394] Antibody [NBP2-29519] - Western blot of mouse heart lysate showing specific immunolabeling of the ~55k HDAC2 protein phosphorylated at Ser394 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Mu, Rt, Hu, Bv, Ch, Gp, Pm, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Histone Deacetylase 2/HDAC2 [p Ser394] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 conjugated to KLH. Accession # Q92769
• Modification: p Ser394
• Specificity: Specific for endogenous levels of the ~55 kDa Histone Deacetylase 2/HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on dephosphopeptide and phosphopeptide affinity columns.
• Predicted Species: Human (100%), Sheep (100%), Chicken (100%), Primate (100%), Bovine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody - Azide Free

• EC 3.5.1.98
• HD2
• HDAC2
• Histone Deacetylase 2
• RPD3
• transcriptional regulator homolog RPD3
• YAF1
• YY1-associated factor 1

Background

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of ser422 and ser424 (EOM et al, 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC2
• Entrez:
  • 3066(Human)
• Uniprot:
  • Q92769(Human)