Histamine H3R Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with transfected cells expressing Histamine receptor H3 at 18 ug/ml.

Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with human brain, neurons and glia at 18 ug/ml.

Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with untransfected HEK293 human embryonic kidney cells.

• Reactivity: Hu, Pm, Pm
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Histamine H3R Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Histamine H3R.
• Epitope: 3rd cytoplasmic domain
• Specificity: Human Histamine H3R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HRH3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 18-36 ug/ml
• Application Notes: .

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Mouse (88%), Rat (88%), Hamster (88%), Bovine (88%), Canine (88%), Equine (88%), Opossum (88%), Guinea pig (88%), Bat (81%), Turkey (81%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histamine H3R Antibody - BSA Free

• GPCR97
• GPCR97G protein-coupled receptor 97
• G-protein coupled receptor 97
• H3R
• HH3R
• Histamine H3 R
• histamine H3 receptor
• Histamine H3R
• histamine receptor H3
• HRH3

Background

H3 Histamine Receptor regulates the release of the neurotransmitters norepinephrine and somatostatin. In histamine-containing neurons of the brain, the H3 receptor acts as a presynaptic autoreceptor, while in nonhistamine-containing neurons in both the central and peripheral nervous systems, it acts as a presynaptic heteroreceptor. Multiple isoforms of H3 receptors are produced by alternative splicing. Histamine H3 receptor has been reported primarily in brain. ESTs have been isolated from brain (normal and cancer), breast cancer, colon cancer, and retinoblastoma libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine H3R Antibody (NLS476)(1)

Publication using NLS476

• Coslovich T, Della Mora A, D'Angelo G, Ortolani F et Al. Histamine H(3) Receptors Expressed in Ventral Horns Modulate Spinal Motor Output Cell Mol Neurobiol 2020-03-27 [PMID: 32211996]

FAQs for Histamine H3R Antibody (NLS476). (Showing 1 - 1 of 1 FAQ).

1. What is the range of dilutions of Histamine receptor H3 Antibody, as primary antibody in IHC experiment.
   • The recommended dilution range is 1:50-1:250. Please keep in mind that all conditions should be optimized by individual researchers and these should serve as only guidelines.

Bioinformatics

• Gene Symbol: HRH3
• Entrez:
  • 11255(Human)
• Uniprot:
  • Q9Y5N1()