F0111). Quadrant markers were set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Human HRH3 or (B) irrelevant protein, and EGFP, was stained with Rabbit Anti-Human HRH3 Monoclonal Antibody (Catalog # MAB102001) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB102001-SP | |||
| Carrier Free | FAB102001V-100UG | |||
| Carrier Free | FAB102001U-100UG | |||
| Carrier Free | FAB102001T-100UG | |||
| Carrier Free | FAB102001S-100UG | |||
| Carrier Free | FAB102001R-100UG | |||
| Carrier Free | FAB102001N-100UG | |||
| Carrier Free | FAB102001G-100UG | |||
F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Histamine H3 R or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Histamine H3 R Monoclonal Antibody (Catalog # MAB10200) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10200-SP | |||
| Carrier Free | FAB10200V-100UG | |||
| Carrier Free | FAB10200U-100UG | |||
| Carrier Free | FAB10200T-100UG | |||
| Carrier Free | FAB10200S-100UG | |||
| Carrier Free | FAB10200R-100UG | |||
| Carrier Free | FAB10200N-100UG | |||
| Carrier Free | FAB10200G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26219-50ul | |||
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons and glia at dilution 7-15 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS3262-img0005.jpg "Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons and glia at dilution 7-15 ug/ml.")
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS3262-img0003.jpg)
![Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-16202_rabbit-histamine-h3r-mab-7h8e6-91220241420131.jpg "Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.")
![Immunoprecipitation: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug Histamine H3R antibody. Western blot was performed from the immunoprecipitate using Histamine H3R antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16202_rabbit-histamine-h3r-mab-7h8e6-912202414154395.jpg)
![Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16202_rabbit-histamine-h3r-mab-7h8e6-912202414213759.jpg)
![Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with transfected cells expressing Histamine receptor H3 at 18 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunocytochemistry-Immunofluorescence-NLS476-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with transfected cells expressing Histamine receptor H3 at 18 ug/ml.")
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with human brain, neurons and glia at 18 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS476-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunocytochemistry-Immunofluorescence-NLS476-img0002.jpg)
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS475] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with human brain, neurons and glia at 2 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS475-img0002.jpg "Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS475] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with human brain, neurons and glia at 2 ug/ml.")
![Western Blot: Histamine H3R Antibody (S04-3I4) [NBP3-14904] - Western blot detection of Histamine H3R in K562, C6, 3T3 cell lysates using Histamine H3R Rabbit mAb (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/Histamine-H3R-Antibody-S04-3I4-Western-Blot-NBP3-14904-img0001.jpg "Western Blot: Histamine H3R Antibody (S04-3I4) [NBP3-14904] - Western blot detection of Histamine H3R in K562, C6, 3T3 cell lysates using Histamine H3R Rabbit mAb (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.")
![Flow Cytometry: Histamine H3R Antibody (27H8) [NBP3-26219] - Overlay Peak curve showing SH-SY5Y cells stained with Histamine H3R Antibody (27H8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26219_rabbit-histamine-h3r-mab-27h8-282202414183626.jpg "Flow Cytometry: Histamine H3R Antibody (27H8) [NBP3-26219] - Overlay Peak curve showing SH-SY5Y cells stained with Histamine H3R Antibody (27H8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
