Hip Antibody (5D11) - Azide and BSA Free

Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11. Analysis of HHIP expression in MCF-7.

Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01] - Analysis of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11 Analysis of HHIP expression in Hela S3 NE.

Western Blot: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.

ELISA: Hip Antibody (5D11) [H00064399-M01] - Detection limit for recombinant GST tagged HHIP is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 5D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hip Antibody (5D11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Hip Antibody (5D11) - Azide and BSA Free (H00064399-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. Anti-Hip Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: HHIP (NP_071920, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQ
• Specificity: HHIP - hedgehog interacting protein
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HHIP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hip Antibody (5D11) - Azide and BSA Free

• FLJ90230
• hedgehog interacting protein
• hedgehog-interacting protein
• HHIP
• Hip
• HIPFLJ20992

Background

This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hip Antibody (H00064399-M01)(4)

Publications using H00064399-M01

• Liu J, Huang J, Lu J, Ouyang R et Al. Obg-like ATPase 1 exacerbated gemcitabine drug resistance of pancreatic cancer iScience 2024-06-17 [PMID: 38883822]
• Miller S, Melen E, Merid SK et al. Genes associated with polymorphic variants predicting lung function are differentially expressed during human lung development. Respir. Res. 2016-07-30 [PMID: 27473260] (IF/IHC, Human)
• Shi Y, Moura U, Opitz I et al. Role of hedgehog signaling in malignant pleural mesothelioma. Clin Cancer Res. 2012-07-11 [PMID: 22733539]
• Yang Q, Shen SS, Zhou S, Ni J, Chen D, Wang G, Li Y. STAT3 activation and aberrant ligand-dependent sonic hedgehog signaling in human pulmonary adenocarcinoma. Exp Mol Pathol. 2012-04-25 [PMID: 22554932] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: HHIP
• Entrez:
  • 64399(Human)
• OMIM:
  • 606178(Human)
• Uniprot:
  • NP_071920()