Hip Antibody (5D11)

Images

 
Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11. Analysis of HHIP expression in MCF-7.
Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01] - Analysis of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11 Analysis of HHIP expression in Hela S3 NE.
Western Blot: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: ...read more
Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.
Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HHIP MaxPab rabbit polyclonal ...read more
ELISA: Hip Antibody (5D11) [H00064399-M01] - Detection limit for recombinant GST tagged HHIP is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clone
5D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Hip Antibody (5D11) Summary

Immunogen
HHIP (NP_071920, 21 a.a. ~ 121 a.a) partial recombinant protein with GST tag.GDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQ
Specificity
HHIP - hedgehog interacting protein
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HHIP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00064399-M01 in the following applications:

  • IHC
    2 publications

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hip Antibody (5D11)

  • FLJ90230
  • hedgehog interacting protein
  • hedgehog-interacting protein
  • HHIP
  • Hip
  • HIPFLJ20992

Background

This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hip Antibody (H00064399-M01)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HHIP
Entrez
OMIM
Uniprot