HIP-55 Antibody - BSA Free

Western Blot: HIP-55 Antibody [NBP1-31026] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 10% SDS PAGE, antibody diluted at 1:1500.

Immunocytochemistry/Immunofluorescence: HIP-55 Antibody [NBP1-31026] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: DBNL protein stained by HIP55 antibody, N-term diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-31026] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HIP-55 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP-55. The exact sequence is proprietary.
• Localization: Cytoplasm, cytoskeleton, Cell projection, lamellipodium
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DBNL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HIP-55 Antibody - BSA Free

• ABP1
• Cervical SH3P7
• CMAP
• drebrin-F
• drebrin-like protein
• drebrin-like
• HIP-55SH3P7Cervical mucin-associated protein
• HPK1-interacting protein of 55 kDa
• SH3 domain-containing protein 7
• src homology 3 domain-containing protein HIP-55

Background

HIP55 possesses a SCAD domain that binds to polymerized actin and an SH3 domain. The latter is suggested to bind to Dynamin 1, a protein involved in the mechanism responsible for the formation of endocytotic vesicles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DBNL