HIP-55 Antibody

Images

 
Western Blot: HIP-55 Antibody [NBP1-31026] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 10% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: HIP-55 Antibody [NBP1-31026] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: DBNL protein stained by HIP55 antibody, N-term diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-31026] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.
Western Blot: HIP-55 Antibody [NBP1-31026] - A: HeLa S3 B: MOLT4 10% SDS PAGE Diluted at 1:1500

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HIP-55 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP55. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton, Cell projection, lamellipodium
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DBNL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIP-55 Antibody

  • ABP1
  • Cervical SH3P7
  • CMAP
  • drebrin-F
  • drebrin-like protein
  • drebrin-like
  • HIP-55SH3P7Cervical mucin-associated protein
  • HPK1-interacting protein of 55 kDa
  • SH3 domain-containing protein 7
  • src homology 3 domain-containing protein HIP-55

Background

HIP55 possesses a SCAD domain that binds to polymerized actin and an SH3 domain. The latter is suggested to bind to Dynamin 1, a protein involved in the mechanism responsible for the formation of endocytotic vesicles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for HIP-55 Antibody (NBP1-31026)

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Bioinformatics

Gene Symbol DBNL