HIGD1A Antibody

Western Blot: HIGD1A Antibody [NBP2-97800] - Anti-HIGD1A rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 15 kDa

Immunohistochemistry-Paraffin: HIGD1A Antibody [NBP2-97800] - Immunochemical staining of human HIGD1A in human rectum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: HIGD1A Antibody [NBP2-97800] - Immunochemical staining of human HIGD1A in human appendix with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: HIGD1A Antibody [NBP2-97800] - HIGD1A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-HIGD1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-HIGD1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 12 kDa

Product Discontinued

HIGD1A Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human HIGD1A fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIGD1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HIGD1A Antibody

• 7420700H20Rik
• DKFZP564K247
• HIG1 domain family member 1A
• HIG1 domain family, member 1A
• HIG1 hypoxia inducible domain family, member 1A
• HIG1DKFZp564K247
• hypoxia inducible gene 1,2210020B17Rik
• Hypoxia-inducible gene 1 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIGD1A