HIBCH Antibody (OTI3H5) - Azide and BSA Free

Western Blot: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Staining of paraffin-embedded Human Kidney tissue using anti-HIBCH mouse monoclonal antibody.

Western Blot: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Human Prostate cancer tissues was stained using HIBCH antibody, showing the up-regulation of HIBCH. This image was submitted via customer Review.

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HIBCH mouse monoclonal antibody.

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Staining of paraffin-embedded Human liver tissue using anti-HIBCH mouse monoclonal antibody.

CyTOF-ready: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HIBCH antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Ca
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HIBCH Antibody (OTI3H5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HIBCH Antibody (OTI3H5) - Azide and BSA Free (NBP2-02673) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIBCH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
• Theoretical MW: 39.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HIBCH Antibody (OTI3H5) - Azide and BSA Free

• 3-hydroxyisobutyryl-CoA hydrolase
• EC 3.1.2.4,3-hydroxyisobutyryl-coenzyme A hydrolase3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
• HIB-CoA hydrolase
• HIBYLCOAH
• HIBYL-CoA-H

Background

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIBCH