HGK/MAP4K4 Antibody (2D4) - Azide and BSA Free

Sandwich ELISA: HGK/MAP4K4 Antibody (2D4) [H00009448-M01] - Detection limit for recombinant GST tagged MAP4K4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HGK/MAP4K4 Antibody (2D4) - Azide and BSA Free Summary

• Immunogen: MAP4K4 (NP_663719, 611 a.a. ~ 710 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPV
• Specificity: MAP4K4 - mitogen-activated protein kinase kinase kinase kinase 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP4K4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HGK/MAP4K4 Antibody (2D4) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• FLH21957
• FLJ10410
• hepatocyte progenitor kinase-like/germinal center kinase-like kinase
• HGK
• HGKFLJ20373
• HPK/GCK-like kinase HGK
• KIAA0687
• MAP4K4
• MAPK/ERK kinase kinase kinase 4
• MEK kinase kinase 4
• MEKKK 4
• MEKKK4
• mitogen-activated protein kinase kinase kinase kinase 4
• Nck-interacting kinase
• NIKFLJ90111

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP4K4
• Entrez:
  • 9448(Human)
• OMIM:
  • 604666(Human)
• Uniprot:
  • NP_663719(Human)