HGD Antibody (SR1835) - BSA Free

Western Blot: HGD Antibody (SR1835) [NBP3-22463] - Western blot analysis using NBP3-22463 in HepG2 cell lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: SR1835
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HGD Antibody (SR1835) - BSA Free Summary

• Description: Novus Biologicals Rabbit HGD Antibody (SR1835) - BSA Free (NBP3-22463) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human HGD (Uniprot #: Q93099)
• Specificity: Detects endogenous levels of total HGD
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HGD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HGD Antibody (SR1835) - BSA Free

• AKU
• EC 1.13.11.5
• HGOFLJ94126
• homogentisate 1,2-dioxygenase (homogentisate oxidase)
• homogentisate 1,2-dioxygenase
• homogentisate oxidase
• Homogentisate oxygenase
• Homogentisic acid oxidase
• homogentisicase

Background

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGD