HGD Antibody (20D12) - BSA Free

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] - Image of HGD Antibody (20D12) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] - Image of HGD Antibody (20D12) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 20D12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HGD Antibody (20D12) - BSA Free Summary

• Description: Novus Biologicals Rabbit HGD Antibody (20D12) - BSA Free (NBP3-26234) is a recombinant monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human HGD [UniProt Q93099]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HGD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HGD Antibody (20D12) - BSA Free

• AKU
• EC 1.13.11.5
• HGOFLJ94126
• homogentisate 1,2-dioxygenase (homogentisate oxidase)
• homogentisate 1,2-dioxygenase
• homogentisate oxidase
• Homogentisate oxygenase
• Homogentisic acid oxidase
• homogentisicase

Background

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGD