HFE Antibody (3F1)

Western Blot: HFE Antibody (3F1) [NBP2-37555] - Western blot analysis using HFE mAb against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: HFE Antibody (3F1) [NBP2-37555] - Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Western Blot: HFE Antibody (3F1) [NBP2-37555] - Western blot analysis using HFE mAb against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)

ELISA: HFE Antibody (3F1) [NBP2-37555] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

HFE Antibody (3F1) Summary

• Immunogen: Purified recombinant fragment of human HFE expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HFE
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for HFE Antibody (3F1)

• dJ221C16.10.1
• hemochromatosis
• hereditary hemochromatosis protein HLA-H
• hereditary hemochromatosis protein
• HFE
• HFE1
• HH
• high Fe
• HLAH
• HLA-H
• HLA-HHFE1
• MGC103790
• MHC class I-like protein HFE
• MVCD7
• TFQTL2

Background

HFE is encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HFE