HEY1 Overexpression Lysate (Native)

Images

 
Western Blot: HEY1 Overexpression Lysate (Adult Normal) [NBL1-11521] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEY1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HEY1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200257 and based on accession number NM_012258. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1, mRNA.
Gene
HEY1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
32.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HEY1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HEY1 Overexpression Lysate (Native)

  • basic helix-loop-helix protein OAF1
  • BHLHb31
  • Cardiovascular helix-loop-helix factor 2
  • CHF-2
  • CHF2hHRT1
  • Class B basic helix-loop-helix protein 31
  • Hairy and enhancer of split-related protein 1
  • hairy/enhancer-of-split related with YRPW motif 1
  • hairy/enhancer-of-split related with YRPW motif protein 1
  • Hairy-related transcription factor 1
  • HERP2HES-related repressor protein 1
  • HESR-1
  • HESR1MGC1274
  • HES-related repressor protein 2
  • HRT1
  • HRT-1OAF1

Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HEY1 Lysate (NBL1-11521) (0)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol HEY1