Hexokinase 2 Antibody

Images

 
Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II protein stained by Hexokinase II antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Paraffin-embedded rat stomach. Hexokinase II antibody diluted at 1:500.
Western Blot: Hexokinase II Antibody [NBP2-16814] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: Hexokinase II Antibody [NBP2-16814] - Paraffin-embedded U87 xenograft. antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Paraffin-embedded mouse muscle. Hexokinase II antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Paraffin-embedded rat kidney. Hexokinase II antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Paraffin-embedded rat heart. Hexokinase II antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.54 mg/ml

Order Details

Hexokinase 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Hexokinase II. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane (By similarity)
Predicted Species
Porcine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (86%), Chicken (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Concentration
0.54 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Hexokinase 2 Antibody

  • DKFZp686M1669
  • EC 2.7.1
  • Hexokinase 2
  • Hexokinase type II
  • HK2
  • HKII
  • HXK2
  • Muscle form hexokinase
  • muscle

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HK2
Entrez
Uniprot