HEXO Antibody (OTI1B3) - Azide and BSA Free

Western Blot: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HEXO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HEXO.

Immunohistochemistry: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - Staining of paraffin-embedded Human Kidney tissue using anti-HEXO mouse monoclonal antibody.

Flow Cytometry: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HEXO monoclonal antibody.

Immunohistochemistry: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HEXO mouse monoclonal antibody.

Immunohistochemistry: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - Staining of paraffin-embedded Human endometrium tissue using anti-HEXO mouse monoclonal antibody.

Immunohistochemistry: HEXO Antibody (OTI1B3) - Azide and BSA Free [NBP2-72312] - Staining of paraffin-embedded Human tonsil using anti-HEXO mouse monoclonal antibody.

• Reactivity: Hu, Rt, Pm, Pm
• Applications: WB, Flow, IHC
• Clone: OTI1B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HEXO Antibody (OTI1B3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HEXO Antibody (OTI1B3) - Azide and BSA Free (NBP2-01655) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ERI1(NP_699163) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 39.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HEXO Antibody (OTI1B3) - Azide and BSA Free

• 3' exoribonuclease
• 3'-5' exonuclease ERI1
• 3'EXO
• 3'HEXO
• EC 3.1
• enhanced RNAi three prime mRNA exonuclease homolog 1
• Eri-1 homolog
• exoribonuclease 1
• HEXO
• histone mRNA 3' end-specific exonuclease
• Histone mRNA 3'-end-specific exoribonuclease
• Histone mRNA 3'-exonuclease 1
• MGC35395
• Protein 3'hExo
• THEX1
• three prime histone mRNA exonuclease 1,3'-5' exoribonuclease 1
• three prime mRNA exonuclease 1

Background

HEXO is a 50-kD protein containing a SAP domain capable of recognizing AT-rich scaffold-attachment regions (SARs) in chromosomal DNA, and a exonuclease domain similar to those of DEDD exonuclease family members. HEXO is an RNA exonuclease that binds to the 3' end of histone mRNAs and probably degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is also able to degrade the 3' overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as a regulator of RNA interference (RNAi).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERI1