HERC5 Antibody - Azide and BSA Free

Western Blot: HERC5 Antibody [NBP3-04987] - Analysis of extracts of various cell lines, using HERC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HERC5 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human HERC5 (NP_057407.2). ELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HERC5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 116 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for HERC5 Antibody - Azide and BSA Free

• CEB1E3 ISG15--protein ligase HERC5
• CEBP1
• cyclin-E binding protein 1
• Cyclin-E-binding protein 1
• EC 6.3.2
• EC 6.3.2.-
• HECT domain and RCC1-like domain-containing protein 5
• hect domain and RLD 5
• HECT E3 ubiquitin ligase
• probable E3 ubiquitin-protein ligase HERC5

Background

The HERC5 gene encodes for a 1,024 amino acid long, 116kDA E3 1SG15-protein ligase HERC5 protein near a cluster of HERC family genes located on chromosome 4. The protein coded for by the HERC5 gene localizes to the cytoplasm and perinuclear region and works as an interferon-induced E3 protein ligase. In doing so, it controlls ISGylation of protein targets. The HERC5 gene has been researched regarding influenza, malaria, teratoma, angelman syndrom, and carcinoma. The HERC5 gene interacts in interferon signaling, cytokine signaling in the immune system, and negative regulation of RIG-I/MDA4 signaling with interactions in NME2, CCNA2, ERCC2, PITX2, and NME1-NME2 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: HERC5