Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 ELISA Kit (Colorimetric)

ELISA: Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 ELISA Kit (Colorimetric) [NBP3-31901] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.063 ng/mL (example only; lot dependent)

Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.063 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HS6ST1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 ELISA Kit (Colorimetric)

• DKFZp547H098
• EC 2.8.2
• EC 2.8.2.-
• FLJ25392
• heparan sulfate 6-O-sulfotransferase 1
• heparan-sulfate 6-sulfotransferase
• HS6ST1
• HS6ST-1
• HS6STheparan-sulfate 6-O-sulfotransferase 1
• MGC116899
• MGC116901

Background

HS6ST1 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. It is expressed in foetal brain and liver and is thought to play a significant role in axonal guidance through sulphation of heparan sulphate proteoglycans, with HS6ST1 expression mirroring that of Slit1 and Slit2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 ELISA Kit (NBP3-31901). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HS6ST1