Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric)

ELISA: Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric) [NBP3-38991] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.125 - 5000 pg/mL (example only; lot dependent)
• Sensitivity: 36 pg/mL (example only; lot dependent)

Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 78.125 - 5000 pg/mL (example only; lot dependent)
• Sensitivity: 36 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HPGDS

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric)

• EC 2.5.1.18
• EC 5.3.99.2
• glutathione S-transferase sigma
• Glutathione S-transferase
• Glutathione-dependent PGD synthase
• Glutathione-requiring prostaglandin D synthase
• GST class-sigma
• GSTS
• GSTShematopoietic prostaglandin D2 synthase
• hematopoietic prostaglandin D synthase
• HPGDS
• H-PGDS
• PGDS
• PGDSglutathione-dependent PGD synthetase
• Prostaglandin-H2 D-isomerase
• PTGDS2

Background

Prostaglandin D synthase catalyzes the isomerization of PGH2 to produce PGD2. Prostaglandin D synthase induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGD synthase have been identified, namely the lipocalin type enzyme (b-trace) and the hematopoietic enzyme. Lipocalin type prostaglandin D synthase is localized in the central nervous system and male genital organs of various mammals and the human heart. This enzyme has been identified as b-trace, which is a major protein in human cerebrospinal fluid. Hematopoietic prostaglandin D synthase is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes. This enzyme, which requires glutathione for activity, belongs to the sigma-class of glutathione-S-transferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (NBP3-38991). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HPGDS