Helios Overexpression Lysate

Images

 
Western Blot: HELIOS Overexpression Lysate (Adult Normal) [NBL1-11892] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HELIOS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Helios Overexpression Lysate Summary

Description

Helios Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206483

Accession#: NM_001079526

Protein Tag: C-MYC/DDK
Gene
IKZF2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Helios Overexpression Lysate

  • Helios
  • IKAROS family zinc finger 2 (Helios)
  • Ikaros family zinc finger protein 2
  • IKZF2
  • MGC34330
  • subfamily 1A, 2 (Helios)
  • zinc finger DNA binding protein Helios
  • zinc finger protein Helios
  • ZNF1A2
  • ZNFN1A2

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. Transcript Variant: This Variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Helios Lysate (NBL1-11892) (0)

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Video Protocols

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Bioinformatics

Gene Symbol IKZF2