Helios Antibody

Images

 
Western Blot: Helios Antibody [H00022807-B01P] - Analysis of IKZF2 expression in transfected 293T cell line by IKZF2 polyclonal antibody. Lane 1: ZNFN1A2 transfected lysate(55 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Helios Antibody [H00022807-B01P] - Analysis of purified antibody to ZNFN1A2 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Helios Antibody Summary

Immunogen
IKZF2 (NP_001072994.1, 1 a.a. ~ 500 a.a) full-length human protein.METEAIDGYITCDNELSPEREHSNMAIDLTSSTPNGQHASPSHMTSTNSVKLEMQSDEECDRKPLSREDEIRGHDEGSSLEEPLIESSEVADNRKVQELQGEGGIRLPNGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRSSLEEHKERCHNYLQNVSMEAAGQVMSHHVPPMEDCKEQEPIMDNNISLVPFERPAVIEKLTGNMGKRKSSTP
Specificity
IKZF2 - IKAROS family zinc finger 2 (Helios),
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
IKZF2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Helios Antibody

  • Helios
  • IKAROS family zinc finger 2 (Helios)
  • Ikaros family zinc finger protein 2
  • IKZF2
  • MGC34330
  • subfamily 1A, 2 (Helios)
  • zinc finger DNA binding protein Helios
  • zinc finger protein Helios
  • ZNF1A2
  • ZNFN1A2

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IKZF2
Entrez
Uniprot