HE4/WFDC2 Antibody (JF62-09)

Images

 
Western Blot: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3
Immunocytochemistry/ Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Flow cytometric analysis of SW480 cells with HE4/WFDC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with ...read more
Immunocytochemistry/ Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JF62-09
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HE4/WFDC2 Antibody (JF62-09) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human HE4.
Localization
Secreted.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
WFDC2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
Application Notes
IP: Use at an assay dependent concentration.
Publications
Read Publication using
NBP2-66883 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HE4/WFDC2 Antibody (JF62-09)

  • dJ461P17.6
  • EDDM4
  • Epididymal secretory protein E4
  • epididymis-specific, whey-acidic protein type, four-disulfide core
  • HE4
  • HE4MGC57529
  • Major epididymis-specific protein E4
  • Putative protease inhibitor WAP5
  • WAP domain containing protein HE4-V4
  • WAP four-disulfide core domain 2
  • WAP four-disulfide core domain protein 2
  • WAP5
  • WAP5epididymal protein 4
  • WFDC2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HE4/WFDC2 Antibody (NBP2-66883)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HE4/WFDC2 Antibody (NBP2-66883). (Showing 1 - 1 of 1 FAQ).

  1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
    • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following link: www.novusbio.com/support/innovators-reward.html

Secondary Antibodies

 

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Bioinformatics Tool for HE4/WFDC2 Antibody (NBP2-66883)

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Diseases for HE4/WFDC2 Antibody (NBP2-66883)

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Bioinformatics

Gene Symbol WFDC2