HDJ2 Antibody (KA2A5.6)

Product Discontinued

HDJ2 Antibody (KA2A5.6) Summary

• Immunogen: N-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein
• Localization: Cytoplasm (ground state). Golgi complex, nucleolus and nuclear membrane (upon heat shock).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNAJA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunoprecipitation 2 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
• Control:

  HDJ2 Overexpression Lysate

Reactivity Notes

E. coli and Monkey. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for HDJ2 Antibody (KA2A5.6)

• dj-2
• DjA1
• DnaJ (Hsp40) homolog, subfamily A, member 1
• dnaJ homolog subfamily A member 1
• DnaJ protein homolog 2
• DNAJ2
• HDJ2
• hDJ-2
• Heat shock 40 kDa protein 4
• Heat shock protein J2
• heat shock protein, DNAJ-like 2
• HSDJ
• HSJ2
• HSJ-2
• HSPF4hdj-2
• Human DnaJ protein 2
• NEDD7
• neural precursor cell expressed, developmentally down-regulated 7

Background

HDJ 2 is a novel human homolog of E. coli's DNA J protein from heat shock protein 40 family. Hsp 40 is involved in chaperonic protein folding. HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the golgi complex, nucleous and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNAJA1
• Entrez:
  • 3301(Human)
  • 15502(Mouse)
  • 65028(Rat)
• Uniprot:
  • P31689(Human)