HDJ2 Antibody (KA2A5.6)

Images

 
Western Blot: HDJ2 Antibody (KA2A5.6) [NB600-952] - Analysis of HDJ-2/DNAJ using HDJ-2/DNAJ Monoclonal Antibody on MAD109 Cells.
Immunohistochemistry-Paraffin: HDJ2 Antibody (KA2A5.6) [NB600-952] - Human tonsil stained with NeoMarkers' HDJ-2 Ab-1 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cells.
Western Blot: HDJ2 Antibody (KA2A5.6) [NB600-952] - Immunoprecipitation and Western Blot of Native and denatured HDJ2 from human cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ba, Ha, Pm, RbSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP, IF
Clone
KA2A5.6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HDJ2 Antibody (KA2A5.6) Summary

Immunogen
N-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein
Localization
Cytoplasm (ground state). Golgi complex, nucleolus and nuclear membrane (upon heat shock).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DNAJA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunoprecipitation 2 ug/ml
  • Immunofluorescence 1:10 - 1:500
Positive Control
HDJ2 Lysate (NBL1-09929)

Reactivity Notes

E. coli and Monkey. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for HDJ2 Antibody (KA2A5.6)

  • dj-2
  • DjA1
  • DnaJ (Hsp40) homolog, subfamily A, member 1
  • dnaJ homolog subfamily A member 1
  • DnaJ protein homolog 2
  • DNAJ2
  • HDJ2
  • hDJ-2
  • Heat shock 40 kDa protein 4
  • Heat shock protein J2
  • heat shock protein, DNAJ-like 2
  • HSDJ
  • HSJ2
  • HSJ-2
  • HSPF4hdj-2
  • Human DnaJ protein 2
  • NEDD7
  • neural precursor cell expressed, developmentally down-regulated 7

Background

HDJ 2 is a novel human homolog of E. coli's DNA J protein from heat shock protein 40 family. Hsp 40 is involved in chaperonic protein folding. HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the golgi complex, nucleous and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DNAJA1
Entrez
Uniprot