Recombinant Human HDHD1A His Protein

SDS-Page: Recombinant Human HDHD1A Protein [NBP1-50883] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human HDHD1A His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-228 of Human HDHD1A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PUDP
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human HDHD1A His Protein

• DXF68S1E5'-PsiMPase
• EC 3.1.3.n6
• family with sequence similarity 16, member A, X-linked
• GS1FAM16AX
• haloacid dehalogenase-like hydrolase domain containing 1
• haloacid dehalogenase-like hydrolase domain containing 1A
• Haloacid dehalogenase-like hydrolase domain-containing protein 1
• Haloacid dehalogenase-like hydrolase domain-containing protein 1A
• HDHD1Apseudouridine-5'-monophosphatase
• Protein GS1

Background

HDHD1A, also known as haloacid dehalogenase-like hydrolase domain-containing protein 1A, belongs to the adiponutrin family. This protein is of interest because it is an X-linked gene that escapes X-inactivation. It is particularly important in the understanding of human X chromosome structural organization as well as the mechanism of X-inactivation. Recombinant human HDHD1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PUDP