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Recombinant Human HDGFRP3 Protein

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SDS-Page: Recombinant Human HDGFRP3 Protein [NBP1-78865] - 15% SDS-PAGE (3ug)
SDS-Page: Recombinant Human HDGFRP3 Protein [NBP1-78865] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human HDGFRP3 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human HDGFRP3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARPRP REYKAGDLVF AKMKGYPHWP ARIDELPEGA VKPPANKYPI FFFGTHETAF LGPKDLFPYK EYKDKFGKSN KRKGFNEGLW EIENNPGVKF TGYQAIQQQS SSETEGEGGN TADASSEEEG DRVEEDGKGK RKNEKAGSKR KKSYTSKKSS KQSRKSPGDE DDKDCKEEEN KSSSEGGDAG NDTRNTTSDL QKTSEGT
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HDGFRP3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human HDGFRP3 Protein

  • HDGF2
  • HDGF-2
  • Hepatoma-derived growth factor 2
  • hepatoma-derived growth factor, related protein 3
  • hepatoma-derived growth factor-related protein 3
  • HRP-3

Background

Hepatoma-derived growth factor, related protein 3, also known as HDGFRP3, is the original member of a family of polypeptides designated HDGF related proteins (HRPs). This protein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is expressed predominantly in the testis and brain, to an intermediate extent in the heart, and to a slight extent in the ovaries, kidneys, spleen, and liver in humans. Recombinant human HDGFRP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Western Blot: HDGF Antibody [NBP1-71926] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-HDGF antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71924 and NBP1-71925, which recognize upstream epitopes.
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF left image, middle image and right image used at a dilution of 1: 1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
LEDGF Antibody
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Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.
(7 Publications)
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Artemin Antibody
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AF1085
Recombinant Mouse Artemin (Catalog # 1085-AR) stimulates proliferation in the SH‑SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Artemin (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Artemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1085). The ND50 is typically 0.1-0.3 µg/mL.
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DUSP5 Antibody
(2F3)
H00001847-M04
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of monoclonal antibody to DUSP5 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Western Blot analysis of DUSP5 expression in PC-12 ( Cat # L012V1 ).
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Frizzled-2 Antibody
NBP2-33842
Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP2-33842] - Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear bodies & cell junctions.
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human testis shows weak to moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CRAT Antibody
NBP1-86616
Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Staining of human lymph node shows no positivity as expected.
Western Blot: CRAT Antibody [NBP1-86616] - Analysis in human cell line TD47D.
Species: Hu
Applications: WB, IHC, IHC-P
HRP-2 Antibody
NBP2-47438
Western Blot: HRP-2 Antibody [NBP2-47438] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: HRP-2 Antibody [NBP2-47438] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: HRP-2 Antibody [NBP2-47438] - Staining of human colon.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
xxxNT-3xxx
[Unconjugated]
267-N3
1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
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Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
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Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Rpn2 Antibody
NBP2-38324
Western Blot: Rpn2 Antibody [NBP2-38324] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38324] - Staining of human kidney.
Immunohistochemistry: Rpn2 Antibody [NBP2-38324] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
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Lysozyme Antibody
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Western Blot: Lysozyme Antibody [NBP2-61118] - Total protein from human cell lines THP-1 and HepG2, human stomach and kidney as well as mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Lysozyme in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-61118] - Analysis of a FFPE tissue section of human kidney with Lysozyme antibody at 1:100 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Lysozyme antibody generated an expected cytoplasmic staining in all the cells with strongest signal in tubular epithelial cells. Some tubules showed more of a punctate staining pattern (vesicular Lysozyme) while all of the tubules depicted a diffused signal (secreted Lysozyme). A subset of cells, especially those from glomeruli, showed nuclear positivity also.
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
GADD45 alpha
Antibody
AF5510
Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45 alpha. PVDF membrane was probed with 1 µg/mL Human GADD45 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for GADD45 alpha was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
WHSC1 Antibody
(CL1063)
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Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human ventricular cancer shows strong nuclear immunoreactivity in tumor cells.
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VEGF-B
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751-VE
VEGF-B [Unconjugated]
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Nbs1 Antibody
NB100-143
Knockout Validated: Nbs1 Antibody [NB100-143] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Nbs1 Polyclonal Antibody (Catalog # NB100-143) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Nbs1 Antibody [NB100-143] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Hela cells. Image from verified customer review.
(8 Reviews)
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Ku80/XRCC5 Antibody
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Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - analysis of HeLa cell lysate (30ug) using anti-Ku80 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-56408] - Human lung adenocarcinoma cell line A549.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-56408] - Human Pancrease (Epithelial cells of acinus indicated with arrows), 4-8ug/ml.
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Proteinase
3/Myeloblastin/PRTN3 ...
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Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.
Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.
(1 Publication)
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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CDC25A Antibody
(336445)
MAB1648
Western blot shows lysates of MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
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Bioinformatics

Gene Symbol HDGFRP3