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Recombinant Human HDGFRP3 Protein

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SDS-Page: Recombinant Human HDGFRP3 Protein [NBP1-78865] - 15% SDS-PAGE (3ug)
SDS-Page: Recombinant Human HDGFRP3 Protein [NBP1-78865] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human HDGFRP3 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human HDGFRP3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARPRP REYKAGDLVF AKMKGYPHWP ARIDELPEGA VKPPANKYPI FFFGTHETAF LGPKDLFPYK EYKDKFGKSN KRKGFNEGLW EIENNPGVKF TGYQAIQQQS SSETEGEGGN TADASSEEEG DRVEEDGKGK RKNEKAGSKR KKSYTSKKSS KQSRKSPGDE DDKDCKEEEN KSSSEGGDAG NDTRNTTSDL QKTSEGT
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HDGFRP3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human HDGFRP3 Protein

  • HDGF2
  • HDGF-2
  • Hepatoma-derived growth factor 2
  • hepatoma-derived growth factor, related protein 3
  • hepatoma-derived growth factor-related protein 3
  • HRP-3

Background

Hepatoma-derived growth factor, related protein 3, also known as HDGFRP3, is the original member of a family of polypeptides designated HDGF related proteins (HRPs). This protein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is expressed predominantly in the testis and brain, to an intermediate extent in the heart, and to a slight extent in the ovaries, kidneys, spleen, and liver in humans. Recombinant human HDGFRP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Immunohistochemistry: HDGF Antibody [NBP1-71926] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF left image, middle image and right image used at a dilution of 1: 1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
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Lysozyme Antibody
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GADD45 alpha
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WHSC1 Antibody
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Nbs1 Antibody
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Proteinase
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Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.
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CDC25A Antibody
(336445)
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Western blot shows lysates of MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Bioinformatics

Gene Symbol HDGFRP3