![Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71924] - Serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-71924-img0003.jpg "Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71924] - Serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 100 and 150 of human Hepatoma-Derived Growth Factor using the numbering given in entry NP_004485.1 (GeneID 3068). |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDGF |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HDGF Antibody (NBP1-71924)Find related products by research area.
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| Gene Symbol | HDGF |
| Uniprot |
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![Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71924] - Serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-71924-img0004.jpg "Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71924] - Serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: HDGF Antibody [NBP1-71924] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HDGF antibody used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71925 and NBP1-71926, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/HDGF-Antibody-Immunoprecipitation-NBP1-71924-img0002.jpg "Immunoprecipitation: HDGF Antibody [NBP1-71924] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HDGF antibody used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71925 and NBP1-71926, which recognize downstream epitopes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")