HDAC6 Antibody

Product Discontinued

HDAC6 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to the C-terminual of Human HDAC6.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of HDAC6 (H1203) pAb in extracts from HeLa cells.
  Immunohistochemistry (IHC) analyzes of HDAC6 (H1203) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HDAC6 Overexpression Lysate

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 27694895).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC6 Antibody

• CPBHM
• EC 3.5.1.98
• FLJ16239
• HD6
• HD6FLJ16239
• HDAC6
• Histone Deacetylase 6
• JM21
• KIAA0901
• PPP1R90

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC6 Antibody (NB100-91805)(5)

Publications using NB100-91805

• Wong Victor S C, Picci Cristina, Swift Michelle et al. a-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein. ENeuro [PMID: 29497702] (WB, WB, WB, Mouse)
• Witt AE, Lee CW, Lee TI et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 2016-10-03 [PMID: 27694895] (WB, Human, Bovine)
• Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. 2016-05-01 [PMID: 27127168] (IHC-P, Human)
• Chopra V, Quinti L, Kim J et al. The Sirtuin 2 Inhibitor AK-7 Is Neuroprotective in Huntington's Disease Mouse Models Cell Rep 2012-11-28 [PMID: 23200855] (WB, Mouse)
• Maxwell MM, Tomkinson EM, Nobles J et al. The Sirtuin 2 microtubule deacetylase is an abundant neuronal protein that accumulates in the aging CNS. Hum Mol Genet. 2011-07-26 [PMID: 21791548] (IF/IHC, WB, Mouse)

Bioinformatics

• Gene Symbol: HDAC6
• Entrez:
  • 10013(Human)
  • 15185(Mouse)
• Uniprot:
  • Q9UBN7(Human)