HDAC6 Antibody (159) - BSA Free

Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in Analysis (intracellular staining).

• Reactivity: Hu, Mu(-), Ze(-)
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 159
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HDAC6 Antibody (159) - BSA Free Summary

• Immunogen: human HDAC6
• Specificity: The mouse monoclonal antibody 159 recognizes SE domain of human histone deacetylase 6 (HDAC6; an intracellular antigen), amino acids 971-981. Crossreactivity with other species was not tested.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1 - 4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 5-10 ug/ml
  • Western Blot 1-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HDAC6 Antibody (159) - BSA Free

• CPBHM
• EC 3.5.1.98
• FLJ16239
• HD6
• HD6FLJ16239
• HDAC6
• Histone Deacetylase 6
• JM21
• KIAA0901
• PPP1R90

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC6