HCN2 Antibody (S71-37)

Images

 
Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37. Load: 15 ug protein. Block: 1.5% BSA ...read more
Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
Immunohistochemistry-Frozen: HCN2 Antibody (S71-37) [NBP2-12895] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Clone
S71-37
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HCN2 Antibody (S71-37) Summary

Immunogen
Fusion protein amino acids 761-863 of rat HCN2
Localization
Membrane
Specificity
Detects approx 95kDa. No cross-reactivity against HCN1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HCN2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of HCN2 Antibody was sufficient for detection of HCN2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HCN2 Antibody (S71-37)

  • BCNG2
  • BCNG-2
  • brain cyclic nucleotide gated channel 2
  • brain cyclic nucleotide-gated channel 2
  • HAC-1
  • hyperpolarization activated cyclic nucleotide-gated potassium channel 2
  • potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Background

1 ug/mL was sufficient for detection of HCN2 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HCN2

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