HADHB Antibody

Product Discontinued

HADHB Antibody Summary

• Immunogen: Synthetic peptide: TILTYPFKNLPT representing the N-Terminus of the human protein (residues 2-13) according to NP_000174.
• Localization: Mitochondrial matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HADHB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA 1:16000
• Application Notes: Western Blot: Preliminary experiments gave no signal but low background in Human Lung and Jurkat extracts at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
• Control:

  HADHB Overexpression Lysate

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Mouse and Dog.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HADHB Antibody

• 2-enoyl-Coenzyme A (CoA) hydratase, beta subunit
• 3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein
• acetyl-CoA acyltransferase
• beta subunit
• beta-ketothiolase
• EC 2.3.1
• EC 2.3.1.16
• ECHB
• hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), beta subunit
• hydroxyacyl-Coenzyme A (CoA) dehydrogenase, beta subunit
• hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
• MGC87480
• mitochondrial trifunctional enzyme, beta subunit
• mitochondrial trifunctional protein, beta subunit
• MTPB
• TP-beta
• trifunctional enzyme subunit beta, mitochondrial

Background

The HADHA and HADHB genes encode the alpha and beta subunits of the mitochondrial trifunctional protein, respectively. The heterocomplex contains 4 alpha and 4 beta subunits and catalyzes 3 steps in the beta-oxidation of fatty acids, including the long-chain 3-hydroxyl-CoA dehydrogenase step. Deficiency of this complex causes sudden unexplained infant death, Reye-like syndrome, cardiomyopathy, or skeletal myopathy. Defects in HADHB are a cause of a variety of diseases such as hypoglycemia, hyperammonemia, mild liver dysfunction and 3-hydroxydicarboxylic aciduria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HADHB
• Entrez:
  • 3032(Human)
• Uniprot:
  • NP_000174.(Human)