HADHB Antibodies
We offer HADHB Antibodies for use in common research applications: Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each HADHB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HADHB Antibodies can be used in a variety of model species: Bovine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Zebrafish. Use the list below to choose the HADHB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HADHB polyclonal antibodies.
Alternate Names for HADHB Antibodies
anti-HADHB antibody, anti-2-enoyl-Coenzyme A (CoA) hydratase, beta subunit antibody, anti-3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein antibody, anti-acetyl-CoA acyltransferase antibody, anti-beta subunit antibody, anti-beta-ketothiolase antibody, anti-EC 2.3.1 antibody, anti-EC 2.3.1.16 antibody, anti-ECHB antibody, anti-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), beta subunit antibody, anti-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, beta subunit antibody, anti-hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit antibody, anti-MGC87480 antibody, anti-mitochondrial trifunctional enzyme, beta subunit antibody, anti-mitochondrial trifunctional protein, beta subunit antibody, anti-MTPB antibody, anti-TP-beta antibody, anti-trifunctional enzyme subunit beta, mitochondrial antibody
7 Results for "hadhb" in Primary Antibodies
![Western Blot: HADHB Antibody [NBP1-82609] - Generation of NIH 3T3 HADHB KO Cell Lines by CRISPR/Cas9(A) Western blot analysis of HADHB protein levels in two HADHB KO clones (AQ and Z) compared with decreasing amounts of total protein extract from WT NIH 3T3 cells. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2162253118303135) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HADHB-Antibody-Knockout-Validated-NBP1-82609-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HADHB Antibody [NBP1-82609] - Generation of NIH 3T3 HADHB KO Cell Lines by CRISPR/Cas9(A) Western blot analysis of HADHB protein levels in two HADHB KO clones (AQ and Z) compared with decreasing amounts of total protein extract from WT NIH 3T3 cells. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2162253118303135) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: HADHB Antibody [NBP1-82609] - (B) IHC staining of murine adductor muscle after ischemia induction revealed expression of HADHB, predominantly in the cytoplasm of cells. (D) Microarray analysis of mRNA expression of HADHB mRNA in the adductor muscle of mice at several time points after induction of ischemia (4 mice per time point). (E) Western blot showing HADHB levels in murine adductor muscle tissue on day 0, 1, and 3 after hindlimb ligation. For each time point, samples from right (R) unligated paws and left (L) ligated paws are presented next to each other. Tubulin was used as a loading control. (F) Quantification of the western blot. The HADHB signal was normalized against tubulin. Each bar represents a biological triplicate and technical duplicate. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2162253118303135) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-NBP1-82609-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: HADHB Antibody [NBP1-82609] - Analysis using Anti-HADHB antibody NBP1-82609 (A) shows similar pattern to independent antibody NBP2-38353 (B).](http://images.novusbio.com/fullsize/HADHB-Antibody-Western-Blot-NBP1-82609-img0010.jpg)
![Western Blot: HADHB Antibody [NBP1-82609] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HADHB-Antibody-Western-Blot-NBP1-82609-img0009.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP1-82609-img0011.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP1-82609-img0012.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP1-82609-img0014.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP1-82609-img0013.jpg)
![Western Blot: HADHB Antibody [NBP1-82609] - Identification of Proteins Binding Pre-miR-329 & Pre-miR-495(A) Schematic representation of RNA pull-down combined with SILAC mass spectrometry. (B) Proteins showing increased or decreased binding to pre-miR-329, pre-miR-495, or both. (C) Western blot validation of HADHB & CIRBP binding to pre-miR-329 & pre-miR-495 under conditions of normal serum & serum starvation. DHX9 is used as a binding control. (D & E) Quantification of the western blot, showing HADHB (D) & CIRBP (E) binding to pre-miR-329 & pre-miR-495 under conditions of normal serum & serum starvation (relative to input). *p < 0.05, n = 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82609_rabbit-polyclonal-hadhb-antibody-310202415392550.jpg)
![Western Blot: HADHB Antibody [NBP1-82609] - Generation of NIH 3T3 HADHB KO Cell Lines by CRISPR/Cas9(A) Western blot analysis of HADHB protein levels in two HADHB KO clones (AQ & Z) compared with decreasing amounts of total protein extract from WT NIH 3T3 cells. (B) Alignment of the region surrounding the CRISPR-Cas9 target sequence from genomic DNA of clones AQ & Z. (C & D) Levels of mature (C) or precursor (D) miR-329 & miR-495 as well as control miR-423 in WT & HADHB KO cell lines quantified by qRT-PCR & normalized to miR-16. For pre-miRs, n = 3; for mature miRs, n = 9. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82609_rabbit-polyclonal-hadhb-antibody-31020241534385.jpg)
![Western Blot: HADHB Antibody [NBP1-82609] - Expression of CIRBP & HADHB In Vivo(A & B) Immunohistochemical staining of murine adductor muscle after ischemia induction revealed expression of both CIRBP (A) & HADHB (B) in these tissues, predominantly in the cytoplasm of cells. (C & D) Microarray analysis of mRNA expression of CIRBP (C) & HADHB (D) mRNA in the adductor muscle of mice at several time points after induction of ischemia (4 mice per time point). (E) Western blot showing HADHB levels in murine adductor muscle tissue on day 0, day 1, & day 3 after hindlimb ligation. For each time point, samples from right (R) unligated paws & left (L) ligated paws are presented next to each other. Tubulin was used as a loading control. (F) Quantification of the western blot. The HADHB signal was normalized against tubulin, & the relative change between ligated & unligated is presented. Each bar represents a biological triplicate & technical duplicate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82609_rabbit-polyclonal-hadhb-antibody-31020241613633.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP2-38353] - Staining in human heart muscle and pancreas tissues using anti-HADHB antibody. Corresponding HADHB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP2-38353-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HADHB Antibody [NBP2-38353] - Staining in human heart muscle and pancreas tissues using anti-HADHB antibody. Corresponding HADHB RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: HADHB Antibody [NBP2-38353] - Analysis using Anti-HADHB antibody NBP2-38353 (A) shows similar pattern to independent antibody NBP1-82609 (B).](http://images.novusbio.com/fullsize/HADHB-Antibody-Western-Blot-NBP2-38353-img0006.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP2-38353] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-NBP2-38353-img0001.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP2-38353] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP2-38353-img0003.jpg)
![Immunohistochemistry-Paraffin: HADHB Antibody [NBP2-38353] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunohistochemistry-Paraffin-NBP2-38353-img0004.jpg)
![Western Blot: HADHB Antibody [NBP2-56259] - Analysis using Anti-HADHB antibody NBP2-56259 (A) shows similar pattern to independent antibody NBP1-82609 (B).](http://images.novusbio.com/fullsize/HADHB-Antibody-Western-Blot-NBP2-56259-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HADHB Antibody [NBP2-56259] - Analysis using Anti-HADHB antibody NBP2-56259 (A) shows similar pattern to independent antibody NBP1-82609 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HADHB Antibody [NBP2-56259] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/HADHB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56259-img0001.jpg)
![Western Blot: HADHB Antibody [NBP1-54750] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/HADHB-Antibody-Western-Blot-NBP1-54750-img0003.jpg "Western Blot: HADHB Antibody [NBP1-54750] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.")
![Immunoprecipitation:HADHB AntibodyNBP3-28899] -Detection of human HADHB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HADHB antibody NBP3-28899 (lot NBP3-28899-1) used for IP at 6 µg per reaction. HADHB was also immunoprecipitated by rabbit anti-HADHB antibody. For blotting immunoprecipitated HADHB, NBP3-28899 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28899_rabbit-hadhb-pab-3012202412392144.jpg "Immunoprecipitation:HADHB AntibodyNBP3-28899] -Detection of human HADHB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HADHB antibody NBP3-28899 (lot NBP3-28899-1) used for IP at 6 µg per reaction. HADHB was also immunoprecipitated by rabbit anti-HADHB antibody. For blotting immunoprecipitated HADHB, NBP3-28899 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:HADHB AntibodyNBP3-28895] -Detection of human HADHB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HADHB antibody NBP3-28895 (lot NBP3-28895-1) used for IP at 6 µg per reaction. HADHB was also immunoprecipitated by rabbit anti-HADHB antibody . For blotting immunoprecipitated HADHB, NBP3-28895 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28895_rabbit-hadhb-pab-301220241745125.jpg "Immunoprecipitation:HADHB AntibodyNBP3-28895] -Detection of human HADHB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HADHB antibody NBP3-28895 (lot NBP3-28895-1) used for IP at 6 µg per reaction. HADHB was also immunoprecipitated by rabbit anti-HADHB antibody . For blotting immunoprecipitated HADHB, NBP3-28895 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: HADHB Antibody [NBP1-30965] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HADHB antibody [N1], N-term (NBP1-30965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30965_rabbit-polyclonal-hadhb-antibody-910202420122050.jpg "Western Blot: HADHB Antibody [NBP1-30965] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HADHB antibody [N1], N-term (NBP1-30965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")