HADHA Antibody

Images

 
Western Blot: HADHA Antibody [NBP1-33588] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HADHA Antibody [NBP1-33588] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HADHA protein stained by HADHA antibody [N2C1], Internal diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-33588] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: HADHA Antibody [NBP1-33588] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: HADHA Antibody [NBP1-33588] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:500.
Immunocytochemistry/ Immunofluorescence: HADHA Antibody [NBP1-33588] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: HADHA protein stained by HADHA antibody, Internal diluted at 1:500. Blue: Hoechst ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.37 mg/ml

Order Details

HADHA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HADHA. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Porcine (92%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HADHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Sheep (89%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.37 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HADHA Antibody

  • 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit
  • 3-oxoacyl-CoA thiolase
  • 78 kDa gastrin-binding protein
  • ECHA
  • gastrin-binding protein
  • GBP
  • HADH
  • hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), alpha subunit
  • hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
  • LCEH
  • LCHAD
  • long-chain 2-enoyl-CoA hydratase
  • long-chain-3-hydroxyacyl-CoA dehydrogenase
  • MGC1728
  • mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit
  • mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alphasubunit
  • mitochondrial trifunctional enzyme, alpha subunit
  • mitochondrial trifunctional protein, alpha subunit
  • MTPATP-ALPHA
  • TP-alpha
  • trifunctional enzyme subunit alpha, mitochondrial

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional HADHA Products

Bioinformatics Tool for HADHA Antibody (NBP1-33588)

Discover related pathways, diseases and genes to HADHA Antibody (NBP1-33588). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for HADHA Antibody (NBP1-33588)

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Bioinformatics

Gene Symbol HADHA