HA-1 Antibody

Product Discontinued

HA-1 Antibody Summary

• Immunogen: Peptide with sequence TLGSCRERQPEFV corresponding to C-Terminus according to NP_036424.2.
• Specificity: To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGAP45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 65-70 kDa band in Jurkat lysate at 1 ug/ml. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 131 kDa according to NP_036424.2.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HA-1 Antibody

• histocompatibility (minor) HA-1
• HLA-HA1
• KIAA0223HA-1
• minor histocompatibility antigen HA-1
• minor histocompatibility protein HA-1

Background

GTPase activator for the Rho-type GTPases (Potential)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA-1 Antibody (NB300-857)(1)

Publication using NB300-857

• den Haan JM, Meadows LM, Wang W et al. The minor histocompatibility antigen HA-1: a diallelic gene with a single aminoacid polymorphism. Science 1998-02-13 [PMID: 9461441]

Bioinformatics

• Gene Symbol: ARHGAP45
• Entrez:
  • 23526(Human)
• Uniprot:
  • NP_036424.(Human)