GULP1/CED-6 Antibody

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat GULP1/CED-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7978) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GULP1/CED-6 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

GULP1/CED-6 Antibody Summary

• Immunogen: E. coli-derived recombinant human GULP1/CED-6
  Met1-Asn92
  Accession # Q9UBP9
• Specificity: Detects human, mouse, and rat GULP1/CED-6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GULP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GULP1/CED-6 Antibody

• CED6
• CED6GULPCED-6
• Cell death protein 6 homolog
• engulfment adapter protein
• FLJ31156
• GULP, engulfment adaptor PTB domain containing 1
• GULP1
• Protein GULP
• PTB domain adapter protein CED-6
• PTB domain adaptor protein CED-6
• PTB domain-containing engulfment adapter protein 1

Background

GULP1 (also PTB domain-containing engulfment adaptor protein 1 and Cell death protein 6 homolog/CED6) is a 36-40 kDa, cytosolic member of the ced-6 family of proteins. It is expressed by multiple cell types, including macrophages, neurons and sinusoidal endothelial cells, and is believed to play a key role in phaogcytosis, particularly involving apoptotic cells. Failure to remove apoptotic cells is suggested to contribute to chronic inflammatory conditions. GULP1 is involved with a MEGF10:GULF:stabilin-1:stabilin-2 internalization pathway. Possibly following endosome formation, it binds to transmembrane activated stabilins and/or LRP1 as a homodimer, and serves as an intermediate for signal transduction. GULP1 also binds to APP, facilitating its proteolysis and the generation of A beta . Human GULP1 is 304 amino acids (aa) in length. It contains one PTB domain (aa 17-154) that interacts with transmembrane receptors, a Leu-zipper (aa 163-193) that mediates dimerization, and a Pro-rich region (aa 238-271) that is likely involved with signal transduction. There are three splice variants. One shows a deletion of aa 31-133, a second contains a 34 aa substitution for aa 134-304, and a third contains a 10 aa substitution for aa 282-304. Over aa 1-92, human GULP1 shares 99% aa sequence identity with mouse GULP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GULP1/CED-6 Antibody (AF7978). (Showing 1 - 1 of 1 FAQ).

1. Is GULP1 localized to mitochondria?
   • I am showing based on localization data from UniProt that it may associate with the cytoplasmic side of the plasma membrane: http://www.uniprot.org/uniprot/Q9UBP9 Additional localization data may be found here at Human protein atlas: http://www.proteinatlas.org/ENSG00000144366

Bioinformatics

• Gene Symbol: GULP1
• Uniprot:
  • Q9UBP9(Human)