GTF2A1 Antibody

Images

 
Western Blot: GTF2A1 Antibody [NBP2-16760] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody [NBP2-16760] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GTF2A1 Antibody [NBP2-16760] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GTF2A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GTF2A1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2A1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus tropicalis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GTF2A1 Antibody

  • General transcription factor IIA subunit 1
  • general transcription factor IIA, 1, 19/37kDa
  • glucose regulated protein, 58kD pseudogene
  • MGC129969
  • MGC129970
  • TF2A1
  • TFIIA alpha, p55
  • TFIIA
  • TFIIA-42
  • TFIIAL
  • transcription initiation factor IIA subunit 1
  • Transcription initiation factor TFIIA 42 kDa subunit

Background

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GTF2A1
Uniprot