GSTA4 Antibody (OTI3F5)

Western Blot: GSTA4 Antibody (OTI3F5) [NBP2-03188] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTA4.

Immunohistochemistry-Paraffin: GSTA4 Antibody (OTI3F5) [NBP2-03188] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GSTA4 mouse monoclonal antibody at 1:150

Flow Cytometry: GSTA4 Antibody (OTI3F5) [NBP2-03188] - Analysis of Hela cells, using anti-GSTA4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: GSTA4 Antibody (OTI3F5) [NBP2-03188] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-GSTA4 mouse monoclonal antibody at 1:150

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: OTI3F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GSTA4 Antibody (OTI3F5) Summary

• Description: Novus Biologicals Mouse GSTA4 Antibody (OTI3F5) (NBP2-03188) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human GSTA4 (NP_001503) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 25.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GSTA4 Antibody (OTI3F5)

• DKFZp686D21185
• EC 2.5.1.18
• glutathione S-alkyltransferase A4
• glutathione S-aralkyltransferase A4
• glutathione S-aryltransferase A4
• glutathione S-transferase A4
• Glutathione S-transferase A4-4
• glutathione S-transferase alpha 4
• glutathione transferase A4-4
• GST class-alpha member 4
• GSTA4-4
• GTA4
• S-(hydroxyalkyl)glutathione lyase A4

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTA4
• Entrez:
  • 2941(Human)