GRPEL1 Antibody - BSA Free

Western Blot: GRPEL1 Antibody - BSA Free [NBP3-04531] - Western blot analysis of lysates from Mouse liver, using GRPEL1 Rabbit pAb (A4999) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] - Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human tonsil tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] - Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human liver tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: GRPEL1 Antibody - BSA Free [NBP3-04531] - Immunofluorescence analysis of HepG2 cells using GRPEL1 Rabbit pAb (A4999) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRPEL1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRPEL1 Antibody - BSA Free (NBP3-04531) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1). MAAQCVRLARRSLPALALSLRPSPRLLCTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRPEL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:200-1:2000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GRPEL1 Antibody - BSA Free

• FLJ25609
• GREPEL1
• grpE protein homolog 1, mitochondrial
• GrpE-like 1, mitochondrial (E. coli)
• GrpE-like protein cochaperone
• HMGEmt-GrpE#1
• Mt-GrpE#1

Background

Essential component of the PAM complex, a complex required for the translocation of transitpeptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems tocontrol the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRPEL1