GRP75/HSPA9B/Mortalin ELISA Development Kit

Product Discontinued

GRP75/HSPA9B/Mortalin ELISA Development Kit Summary

• Description: Grp75 ELISA development set contains sufficient reagents for the development of 5 x 96 well ELISA plates which provides analysis of ~200 samples in duplicate
• Specificity: This assay detects Grp75 (Mortalin, HSPA9) in cell lysates. Cross reactivity with related HSP70 family members is <0.03% for DnaK, Hsc70, Bip, HSP70, and <0.078% for HSP90, Grp94, and HSP60.
• Standard Curve Range: 3.125 - 100 ng/ml
• Sensitivity: 0.762 ng/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: N/A
• Intra-Assay: N/A
• Spike Recovery: N/A
• Sample Volume: 100 uL
• Kit Type: ELISA Development Kit
• Gene: HSPA9

Applications/Dilutions

• Dilutions:
  • ELISA

Reactivity Notes

This assay detects Grp75 (Mortalin, HSPA9) in cell lysates. Cross reactivity with related HSP70 family members is <0.03% for DnaK, Hsc70, Bip, HSP70, and <0.078% for HSP90, Grp94, and HSP60.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.

Alternate Names for GRP75/HSPA9B/Mortalin ELISA Development Kit

• 75 kDa glucose-regulated protein
• CSA
• GRP75
• GRP-75
• GRP75mthsp75
• Heat shock 70 kDa protein 9
• heat shock 70kD protein 9B
• heat shock 70kDa protein 9 (mortalin)
• heat shock 70kDa protein 9B (mortalin-2)
• HSPA9
• HSPA9B
• HSPA9BMGC4500
• Mortalin
• mortalin, perinuclear
• Mortalin-2
• MOT
• MOT2
• mot-2
• MTHSP75
• p66-mortalin
• PBP74
• PBP74CSA
• Peptide-binding protein 74
• stress-70 protein, mitochondrial

Background

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: HSPA9