Recombinant Human Growth Hormone Protein

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SDS-Page: Human Growth Hormone Protein [NBP2-34890]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Recombinant Human Growth Hormone Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 191 residues of GH1.

Source: E. coli

Amino Acid Sequence: FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Growth Hormone protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 107 IU/mg
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GH1
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution with 20mM PB, pH 7.0, with 3% Mannitol.
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human Growth Hormone Protein

  • GH1
  • GHIGHD1B
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • Growth Hormone
  • hGH-N
  • Somatotropin

Background

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) as well as by signals from the periphery-ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (a.a.) residue precursor protein with a 26 a.a. putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for Growth Hormone Protein (NBP2-34890). (Showing 1 - 1 of 1 FAQ).

  1. What is the expiration date of this product?
    • We guarantee this product for 2 years from the date of receipt.

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Blogs on Growth Hormone.

Growth hormone (GH, somatotropin, hGH, pituitary growth hormone)
GH is a member of the large family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. Additionally, GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic...  Read full blog post.

Somatostatin Receptor 2: Treating Patients Who Cannot Stop Growing
Acromegaly is a rare life-shortening disease caused by elevated levels of growth hormone (GH) secreted by a tumor on the pituitary gland. Treatments include somatostatin analogs, which activate somatostatin receptor 2 (SSTR2), reducing GH secretion an...  Read full blog post.

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Bioinformatics

Gene Symbol GH1
Entrez
Uniprot