Human GRO2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Human GRO2 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The CXCL2 Prom/LUCPorter™ reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with the CXCL2 induction-related signaling pathways. CXC-motif chemokine ligand 2 (CXCL2) is a small cytokine that belongs to the C-X-C chemokine family and is also known as macrophage inflammatory protein 2 (MIP-2). CXCL2 is one of the major proinflammatory cytokines which is induced by innate immune receptors such TLRs and NLRs and also mediates LPS-induced osteoclastogenesis. The CXCL2 induction by phorbol 12-myristate 13-acetate (PMA) as well as flagellin is shown in Figures 1 and 2. In the test, PMA and flagellin induced the CXCL2 promoter activity in a dose response manner, which was quantified by luciferase activity (Figures 1 and 2).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The CXCL2 stable reporter cell line is a stably transfected HEK 293 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the CXCL2 promoter.
• Target Species: Human
• Specificity: CXCL2 Prom/LUCPorter™ (HEK 293)
• Selection Agent: Puromycin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Host: HEK293
• Gene: CXCL2

Applications/Dilutions

• Dilutions:
  • Ligand Activation
  • Proximity Ligation Assay
• Agonist:
  • Flagellin, TLR5 Ligand NBP2-25289
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the CXCL2 reporter cell line.
- Wash hands after handling the CXCL2 reporter cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with the cell line.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human GRO2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• GRO2 oncogene
• GRO2MIP2
• GROb
• gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MIP-2a
• MIP2AC-X-C motif chemokine 2
• SCYB2MIP2-alpha

Background

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatorychemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highlyenhanced hematopoietic activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCL2