GRB2 Antibody (669604) - (SH2 Domain)

Western blot shows lysates of HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRB2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 669604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GRB2 Antibody (669604) - (SH2 Domain) Summary

• Immunogen: E. coli-derived recombinant human GRB2 (SH2 Domain)
  Trp60-Glu152
  Accession # P62993
• Specificity: Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRB2 Antibody (669604) - (SH2 Domain)

• abundant SRC homology
• Adapter protein GRB2
• ASH
• EGFRBP-GRB2
• epidermal growth factor receptor-binding protein GRB2
• GRB2
• Grb3-3
• growth factor receptor-bound protein 2
• growth factor receptor-bound protein 3
• HT027
• MST084
• MSTP084
• NCKAP2
• Protein Ash
• SH2/SH3 adapter GRB2

Background

GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules.  It serves as a linker for many intracellular proteins.  Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors.  Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation.  Human GRB2 is 217 amino acids (aa) in length.  It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212).  SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions.  There is one splice form that shows a deletion of aa 60-100.  This removes the SH2 domain and initiates apoptosis.  Human GRB2 is over 99% aa identical to mouse and dog GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRB2 Antibody (MAB38461)(2)

Publications using MAB38461

• JA Jadwin, D Oh, TG Curran, M Ogiue-Iked, L Jia, FM White, K Machida, J Yu, BJ Mayer Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases Elife, 2016-04-12;5(0):. 2016-04-12 [PMID: 27071344] (Western Blot, Human)
• Cheng S, Sun G, Schlaepfer D, Pallen C Grb2 promotes integrin-induced focal adhesion kinase (FAK) autophosphorylation and directs the phosphorylation of protein tyrosine phosphatase alpha by the Src-FAK kinase complex. Mol Cell Biol, 2013-11-18;34(3):348-61. 2013-11-18 [PMID: 24248601] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GRB2
• Uniprot:
  • P62993()