GRASP65 Antibody (JB36-34)

Western Blot: GRASP65 Antibody (JB36-34) [NBP2-75516] - Analysis of GRASP65 on different cell lysates using anti-GRASP65 at 1/500 dilution.Positive control:Line 1: THP-1 Line 2: SKBR3

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (JB36-34) [NBP2-75516] - Staining GRASP65 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GRASP65 Antibody (JB36-34) [NBP2-75516] - Analysis of paraffin-embedded human uterine tissue using anti-GRASP65 antibody. Counter stained with hematoxylin.

Flow Cytometry: GRASP65 Antibody (JB36-34) [NBP2-75516] - Analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (JB36-34) [NBP2-75516] - Staining GRASP65 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (JB36-34) [NBP2-75516] - Staining GRASP65 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GRASP65 Antibody (JB36-34) [NBP2-75516] - Analysis of paraffin-embedded human colon cancer tissue using anti-GRASP65 antibody. Counter stained with hematoxylin.

Product Discontinued

GRASP65 Antibody (JB36-34) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human GRASP65 aa 396-440 / 440. (SwissProt: Q9BQQ3 Human)
• Localization: Golgi apparatus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GORASP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GRASP65 Antibody (JB36-34)

• Golgi peripheral membrane protein p65
• Golgi phosphoprotein 5MGC118897
• golgi reassembly stacking protein 1, 65kDa
• Golgi reassembly-stacking protein 1
• Golgi reassembly-stacking protein of 65 kDa
• GRASP65MGC118894
• P6565 kDa

Background

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GORASP1