Granzyme B Antibody

Immunohistochemistry-Paraffin: Granzyme B Antibody [NB110-59915] - Formalin fixed paraffin embedded human Hodgkin's lymphoma stained with Granzyme B antibody (NB110-59915).

Immunohistochemistry-Paraffin: Granzyme B Antibody [NB110-59915] - ab4069 at 1/20 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.

Product Discontinued

Granzyme B Antibody Summary

• Description: This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTLs or NK cells.
  
  This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.
• Immunogen: A synthetic peptide from the N-terminus of human Granzyme B.
• Localization: Cytoplasmic
• Specificity: This antibody reacts with a 32 kD protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GZMB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for Granzyme B Antibody

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GZMB
• Entrez:
  • 3002(Human)
  • 14939(Mouse)
  • 171528(Rat)