GPRC5A/RAI3 Antibody (481906) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) Human GPRC5A or (B) irrelevant protein and eGFP, was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, Flow
• Clone: 481906
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPRC5A/RAI3 Antibody (481906) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPRC5A
  Met1-Ser357
  Accession # Q8NFJ5
• Specificity: Stains human GPRC5A transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPRC5A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPRC5A/RAI3 Antibody (481906) [Unconjugated]

• G protein-coupled receptor, family C, group 5, member A
• GPCR5A
• GPRC5A
• Orphan G-protein-coupling receptor PEIG-1
• RAI3
• RAI3G-protein coupled receptor family C group 5 member A
• RAIG1
• RAIG1RAIG-1
• retinoic acid induced 3
• retinoic acid responsive
• Retinoic acid-induced gene 1 protein
• retinoic acid-induced protein 3

Background

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPRC5A/RAI3 Antibody (MAB5239)(1)

Publication using MAB5239

• PS Caceres, D Gravotta, PJ Zager, N Dephoure, E Rodriguez- Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins Proc. Natl. Acad. Sci. U.S.A., 2019-05-29;116(24):11796-11805. 2019-05-29 [PMID: 31142645] (Western Blot, Canine)

Bioinformatics

• Gene Symbol: GPRC5A
• Uniprot:
  • Q8NFJ5()