GPR39 Antibody - BSA Free

Images

 
Western Blot: GPR39 Antibody [NLS139] - Western blot analysis of GPR39 in human small intestine lysate.
Immunohistochemistry: GPR39 Antibody [NLS139] - Staining of human brain, neurons, and glia.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.1 mg/ml

Order Details

GPR39 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the human GPR39 protein (within residues 150-200). [Swiss-Prot O43194]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPR39
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 2 ug/ml
Application Notes
This GPR39 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed at ~50 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 29079520).
Publications
Read Publications using
NLS139 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR39 Antibody - BSA Free

  • G protein-coupled receptor 39
  • GPR39
  • G-protein coupled receptor 39
  • MGC149541

Background

GPR39 (G-protein coupled receptor 39) is an orphan GPCR receptor from ghrelin/motilin receptor subfamily of G-protein coupled receptor 1 family, and it is constitutively active with Zn2+ as its physiological agonist. GPR39 is a multipass transmembrane protein found in cell membranes and is expressed in several tissues such as stomach, intestine and hypothalamus. Obestatin, a 23 amino acid peptide originating from ghrelin precursor, was originally shown to be the endogenous ligand of GPR39, a study that happened to be non-reproducible, and later on it was shown that obestatin does not bind, nor activate GPR39 but that Zn2+ could stimulate GPR39 signalling in vitro, thereby inferring that obestatin is not the natural ligand of GPR39. GPR39 receptor also displays a strong ligand-independent signaling activity through G-alpha12/13 as well as G-alphaq. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. GPR39 has been shown to implicate in the regulation of insulin release/pancreatic function, body weight, gastrointestinal mobility, hormone secretion and cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR39 Antibody (NLS139)(4)

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Product General Protocols

View specific protocols for GPR39 Antibody (NLS139): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GPR39 Antibody (NLS139) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GPR39
Entrez
Uniprot