GPR10 Antibody

Product Discontinued

GPR10 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human GPR10.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRLHR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of GPR10 (Q223) pAb in extracts from Jurkat cells. Immunohistochemistry was reported in scientific literature.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR10 Antibody

• G protein-coupled receptor 10
• GPR10
• GPR10MGC126539
• G-protein coupled receptor 10
• GR3
• hGR3
• MGC126541
• PRLHR
• prolactin releasing hormone receptor
• prolactin releasing peptide receptor
• prolactin-releasing hormone receptor
• prolactin-releasing peptide receptor
• PrRP receptor
• PrRPR

Background

G protein-coupled receptors (GPRs or GPCRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, are members of the largest protein family and play a role in many different stimulus-response pathways. G protein-coupled receptors mediate extracellular signals into intracellular signals (G protein activation). They respond to a great variety of signaling molecules, including hormones, neurotransmitters and other proteins and peptides. GPR proteins are integral seven-pass membrane proteins with some conserved amino acid regions. G protein coupled receptor 10 (GPR10) acts as a receptor for prolactin-releasing peptide (PrRP). GPR10 plays a role in the regulation of food intake, pain-signal processing and in lactation. Primarily expressed in pituitary gland, it is repressed by bromocriptine. GPR10 interacts with various other proteins, including GRIP1, GRIP2 and PICK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR10 Antibody (NBP1-00854)(1)

Publication using NBP1-00854

• Varghese BV, Koohestani F, McWilliams M et al. Loss of the repressor REST in uterine fibroids promotes aberrant G protein-coupled receptor 10 expression and activates mammalian target of rapamycin pathway. Proc Natl Acad Sci U S A 2013-02-05 [PMID: 23284171] (IF/IHC, ICC/IF, WB, Human, Mouse)

Bioinformatics

• Gene Symbol: PRLHR
• Entrez:
  • 2834(Human)
  • 246075(Rat)