gp130/CD130 Antibody (125623) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

gp130 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013)and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL-6 deficiency leads to decreased gp130 protein in the retina. (A) Fold difference of Gp130 mRNA in the IL-6−/− retina normalized to WT Gp130 mRNA (dotted line) via the delta delta Ct method. (B) Densitometry analysis (right) of gp130 and beta -actin immunoblotting (left) in WT (white) and IL-6−/− (gray) retina.; student’s t-test. Error bars indicates STDEV. (C–D) Whole mount (C) and sagittal cross sections (D) of WT (left) and IL-6−/− (right) retina reveal co-localization (arrowheads; C and arrows; D) of gp130 immunolabeling (green) with beta -Tubulin+ RGCs. Error bars represent standard deviation and asterisks indicate p<0.05 (A,B). Scale bars = 20 μm (C,D). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27747134), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 125623
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

gp130/CD130 Antibody (125623) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse gp130
  Gln23-Glu617 (predicted)
  Accession # Q6PDI9
• Specificity: Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL6ST
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody (125623) [Unconjugated]

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

1. Narazaki, M. et al. (1993) Blood 82:1120.
2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gp130/CD130 Antibody (MAB4681)(10)

Publications using MAB4681

• Nancy M. Lainez, Djurdjica Coss Leukemia Inhibitory Factor Represses GnRH Gene Expression via cFOS during Inflammation in Male Mice Neuroendocrinology 1/10/2019 [PMID: 30630179]
• AN Wilkinson, KH Gartlan, G Kelly, LD Samson, SD Olver, J Avery, N Zomerdijk, SK Tey, JS Lee, S Vuckovic, GR Hill Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130 J. Immunol., 2018-04-06;0(0):. 2018-04-06 [PMID: 29626088] (Flow Cytometry, Mouse)
• S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling J. Biol. Chem, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280243] (Neutralization, Mouse)
• C Li, Y Bi, Y Li, H Yang, Q Yu, J Wang, Y Wang, H Su, A Jia, Y Hu, L Han, J Zhang, S Li, W Tao, G Liu Dendritic cell MST1 inhibits Th17 differentiation Nat Commun, 2017-02-01;8(0):14275. 2017-02-01 [PMID: 28145433] (Flow Cytometry, Mouse)
• Franklin D. Echevarria, Abigayle E. Rickman, Rebecca M. Sappington Interleukin-6: A Constitutive Modulator of Glycoprotein 130, Neuroinflammatory and Cell Survival Signaling in Retina Journal of Clinical & Cellular Immunology 10/18/2016 [PMID: 27747134]
• FD Echevarria, CC Walker, SK Abella, M Won, RM Sappington Stressor-dependent Alterations in Glycoprotein 130: Implications for Glial Cell Reactivity, Cytokine Signaling and Ganglion Cell Health in Glaucoma J Clin Exp Ophthalmol 6/30/2013 [PMID: 25018894]
• Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts. Cytokine, 2014-04-22;68(2):101-9. 2014-04-22 [PMID: 24767864] (IHC-P, Mouse)
• Bailey , Charles, Huang , I-Chueh, Kam , Christin, Farzan , Michael Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog, 2012-09-06;8(9):e1002909. 2012-09-06 [PMID: 22969429] (Flow Cytometry, Mouse)
• Matsumoto S, Hara T, Mitsuyama K, Yamamoto M, Tsuruta O, Sata M, Scheller J, Rose-John S, Kado S, Takada T Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model. J. Immunol., 2009-12-30;184(3):1543-51. 2009-12-30 [PMID: 20042582] (IHC-Fr, Mouse)
• TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3. Am. J. Physiol., Cell Physiol., 2007-11-21;294(1):C306-12. 2007-11-21 [PMID: 18032527] (ICC, Western Blot, Rat)

Bioinformatics

• Gene Symbol: IL6ST
• Uniprot:
  • Q6PDI9()