GOT2 Antibody

Western Blot: GOT2 Antibody [NB100-93404] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GOT2 Antibody Summary

• Description: Novus Biologicals Goat GOT2 Antibody (NB100-93404) is a polyclonal antibody validated for use in WB and ELISA. Anti-GOT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CKDADEAKRVES corresponding to internal region according to NP_002071.2.
• Epitope: CKDADEAKRVES
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GOT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in human kidney and liver lysates (calculated MW of 47.5 kDa band according to NP_002071.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GOT2 Antibody

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOT2 Antibody (NB100-93404)(1)

Publication using NB100-93404

• Teichberg VI. From the liver to the brain across the blood-brain barrier. Proc Natl Acad Sci U S A 2007-05-01 [PMID: 17463093]

Bioinformatics

• Gene Symbol: GOT2
• Entrez:
  • 2806(Human)
  • 14719(Mouse)
  • 25721(Rat)
• Uniprot:
  • NP_002071.2(Human)