GOT2 Antibody (4H8) - Azide and BSA Free

Western Blot: GOT2 Antibody (4H8) [H00002806-M09] - Analysis of GOT2 expression in HepG2 (Cat # L019V1).

Sandwich ELISA: GOT2 Antibody (4H8) [H00002806-M09] - Detection limit for recombinant GST tagged GOT2 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GOT2 Antibody (4H8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GOT2 (NP_002071, 331 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LNTPDLRKQWLQEVKGMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK
• Specificity: Reacts with glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GOT2 Antibody (4H8) - Azide and BSA Free

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOT2
• Entrez:
  • 2806(Human)
• Uniprot:
  • NP_002071(Human)