GOLM1 Antibody (3B10)

Western Blot: GOLM1 Antibody (3B10) [H00051280-M04] - Analysis of GOLM1 expression in transfected 293T cell line by GOLPH2 monoclonal antibody (M04), clone 3B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: GOLM1 Antibody (3B10) [H00051280-M04] - Analysis of GOLM1 transfected lysate using anti-GOLM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GOLM1 rabbit polyclonal antibody.

ELISA: GOLM1 Antibody (3B10) [H00051280-M04] - Detection limit for recombinant GST tagged GOLM1 is 0.1 ng/ml as a capture antibody.

Product Discontinued

GOLM1 Antibody (3B10) Summary

• Immunogen: GOLM1 (NP_057632, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL
• Specificity: GOLPH2 - golgi phosphoprotein 2 (3B10)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GOLM1 Antibody (3B10)

• bA379P1.3
• FLJ23608
• golgi membrane protein 1
• Golgi membrane protein GP73
• Golgi phosphoprotein 2C9orf155
• golgi protein, 73-kD
• GOLPH2 FLJ22634
• GP73 chromosome 9 open reading frame 155
• GP73
• PSEC0257

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLM1 Antibody (H00051280-M04)(2)

Publications using H00051280-M04

• Liu Y, Zhang X, Zhou S et al. Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase-2 in hepatocellular carcinoma cells and inhibits cell invasion. J Cell Mol Med. 2019-05-08 [PMID: 30677226]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: GOLM1
• Entrez:
  • 51280(Human)
• Uniprot:
  • NP_057632(Human)